|Title||Detection and Quantification of Enteric Pathogens in Aerosols Near Open Wastewater Canals in Cities with Poor Sanitation.|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||O Ginn, L Rocha-Melogno, A Bivins, S Lowry, M Cardelino, D Nichols, SN Tripathi, F Soria, M Andrade, M Bergin, MA Deshusses, and J Brown|
|Journal||Environmental Science & Technology|
|Pagination||14758 - 14771|
Urban sanitation infrastructure is inadequate in many low-income countries, leading to the presence of highly concentrated, uncontained fecal waste streams in densely populated areas. Combined with mechanisms of aerosolization, airborne transport of enteric microbes and their genetic material is possible in such settings but remains poorly characterized. We detected and quantified enteric pathogen-associated gene targets in aerosol samples near open wastewater canals (OWCs) or impacted (receiving sewage or wastewater) surface waters and control sites in La Paz, Bolivia; Kanpur, India; and Atlanta, USA, via multiplex reverse-transcription qPCR (37 targets) and ddPCR (13 targets). We detected a wide range of enteric targets, some not previously reported in extramural urban aerosols, with more frequent detections of all enteric targets at higher densities in La Paz and Kanpur near OWCs. We report density estimates ranging up to 4.7 × 10<sup>2</sup> gc per m<sub>air</sub><sup>3</sup> across all targets including heat-stable enterotoxigenic <i>Escherichia coli</i>, <i>Campylobacter jejuni</i>, enteroinvasive <i>E. coli</i>/<i>Shigella</i> spp., <i>Salmonella</i> spp., norovirus, and <i>Cryptosporidium</i> spp. Estimated 25, 76, and 0% of samples containing positive pathogen detects were accompanied by culturable <i>E. coli</i> in La Paz, Kanpur, and Atlanta, respectively, suggesting potential for viability of enteric microbes at the point of sampling. Airborne transmission of enteric pathogens merits further investigation in cities with poor sanitation.
|Short Title||Environmental Science & Technology|